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embryo fibroblast中文

發音:   用"embryo fibroblast"造句
  • 胚胎成纖維細胞
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  1. Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast
    66弱毒株在雞胚成纖維細胞上的培養
  2. Development of chicken embryo fibroblast cell line resistant to j subgroup avian leukosis virus infection
    亞群禽白血病病毒的雞胚成纖維細胞系建立
  3. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f . for the construction of transfer vector pfgs11haf , aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11 . recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l . recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier
    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ;將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點,通過酶切鑒定獲得了pfgs11ha ;將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ,再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平,后再共同用sac酶切連接得puc18pelf , sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點,通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  4. Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然后收獲其免疫脾細胞并與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆并通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。
  5. From dead chicken which infected infectious stunting syndrom of our province , one virue was isolated using spfeggs , chicken embryo fibroblast , mdck18 , and vero cell . this virus was unable to agglutinate chicken erythrocytes . in order to definite the pathogeny of infectious stunting syndrom . physical and chemical specific property , types of the nucleic acid of the isolated virus , recurrent infection and other biological property determination and indirect elisa test proved it as a parvoviruses like strain of chicken
    為確定該病的病原,對所分離病毒進行了理化特性測定、病毒核酸型別測定、動物回歸試驗等生物學特性測定,證明該分離病毒與細小病毒科( parvovirdae )細小病毒屬( parvovirus )的雞細小病毒( chickenparvovirus )特性基本相符,核酸型為dna型。

相關詞匯

  1. embryo dormancy中文
  2. embryo engineering中文
  3. embryo esker中文
  4. embryo extract中文
  5. embryo extract apparatus中文
  6. embryo fisheries中文
  7. embryo fusion中文
  8. embryo grafting中文
  9. embryo growth development factor中文
  10. embryo growth retardation中文
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