- In bacterial , many enzymes compete for intracellular pep , especially the phosphotransferase system which is responsible for glucose transport in e . coli . this system uses pep as a phosphate donor and converts it to pyruvate , which is less likely to recycle back to pep
- Tpt1 is essential for the vegetative growth of s . cerevisiae and codes for the trna 2 ' - phosphotransferase by using the plasmid shuffling technique , we have constructed a tpt1 - deleted strain and transformed it with trpt1 gene and found that the trpt1 could complement the yeast tpt1
Phosphotransferases are a category of enzymes (EC number 2.7) that catalyze phosphorylation reactions.