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毒性基因英文

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"毒性基因"怎麼讀用"毒性基因"造句

英文翻譯手機手機版

  • toxophore
  • virulent gene

例句與用法

  • In this study , the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi , xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing , the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully . after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e . coli , the transformed hosts were induced by iptg , bysds - page and elisa analysis of host protein . the expression of the objective gene was detected , and it could account for 16 . 28 % of the total host protein . inclusion body was prepared from the incubating expression hosts induced by iptg
    同時將原核表達載體pet - 28c用nco , xho雙酶切,回收酶切產物,將回收的酶切產物pea , h3 ,載體進行連接,并轉入dh5感受態細胞內,培養12 - 18小時后,挑取陽性菌落,經nco , xho雙酶切分析及pcr檢測,篩選到陽性克隆,其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。
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